NovelCyto^®Microwell Single-Cell Capture Platform

NovelCyto^®Microwell Single-Cell Capture Platform is a complete single-cell transcriptome solution developed by NovelBio, integrating hardware instruments, capture beads, specialized chips, barcoding reagents, and library preparation components.

Technical Principle

The NovelCyto® Microwell Single-Cell Capture Platform utilizes a proprietary microwell array system to isolate and capture individual cells. Specifically, when a cell suspension is loaded into the microchip, both the cells and magnetic beads settle independently into distinct microwells via gravitational settling, achieving highly efficient single-cell separation and capture.

Technical Advantages

1. Flexible Handling – Broad Tissue Compatibility The NovelCyto® Single-Cell Capture Platform supports a wide range of tissue types, including samples with low cell input (≥30,000 cells) or challenging preparations such as high debris content. It also maintains high capture efficiency for samples with reduced cell viability, ensuring reliable performance across diverse experimental conditions.

2. Superior Data Quality – Proprietary Barcoded Beads Through in-house probe design and bead chemistry optimization, we have enhanced cell barcode recognition, significantly lowering background noise in sequencing data and improving mRNA capture efficiency.

Performance Metrics: · Cells Captured: 14,519 and 15,524 cells in two independent runs.
· Mean Reads per Cell: ~20,000, meeting standard sequencing depth requirements.
· Key QC Parameters: High median genes per cell, elevated UMI counts, and consistently high sequencing saturation—demonstrating robust and reproducible data output.

3. Distinct Cell Clustering & Exceptional Reproducibility

Analysis of mouse lung tissues reveals comprehensive and well-resolved cell types, including detailed subpopulations of immune, epithelial, and stromal cells. The closely matched cell type proportions across replicate experiments validate the platform’s high experimental reproducibility.

Summary The NovelCyto® Microwell Single-Cell Capture Platform delivers high-performance results across essential metrics—including cell recovery, gene detection, UMI counts, mapping rates, and inter-run consistency—ensuring accurate transcriptional profiling and providing a cost-effective solution for single-cell RNA sequencing.

1. NovelCyto v1.0: Complete Documentation of the Experimental Procedure
2. NovelCyto v1.0 Single-Cell Experimental Data Presentation
3. Preparation and Capture of Single-Cell Suspension from Normal Mouse Pancreatic Tissue
4. Unraveling the Single-Cell Mysteries of Subcutaneous Bladder Tumor Tissue in Mice
5. Single-Cell Profiling of Mouse Lung Tissue
6. Neurons Survive! NovelBio's Tissue Dissociation Breakthrough Preserves Viable Neurons from Brain Tissue.